factor xa protease (New England Biolabs)

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New England Biolabs factor xa protease
Factor Xa Protease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 740 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/factor+xa+protease/Factor+Xa+Protease/pm42129495-314-9-12
Average 96 stars, based on 740 article reviews

factor xa protease - by Bioz Stars, 2026-09

96/100 stars

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Article Title: Production of Xylooligosaccharides (XOSs) with Anti-inflammatory and Prebiotic Activities Using Recombinant Endo-1,4-β-xylanases from Bacillus subtilis
Article Snippet: To remove MBP from the MBP- Bs XynA11 fusion protein, factor Xa protease was used according to the manufacturer’s protocol (#P8010S, NEB).

Article Title: Production of Xylooligosaccharides (XOSs) with Anti-inflammatory and Prebiotic Activities Using Recombinant Endo-1,4-β-xylanases from Bacillus subtilis .
Article Snippet: The enzymatic production of xylooligosaccharides (XOSs) from xylan-rich agricultural byproducts has gained attention as a sustainable and environmentally friendly approach.. In this work, two forms of recombinant Bacillus subtilis xylanases, MBP-BsXynA11 (a fusion of a maltose-binding protein with the xylanase) and BsXynA11-His (the xylanase with a 10xHis-tag), were investigated for their ability to convert xylan into value-added XOS.. Both enzymes exhibited similar temperature and pH optima at 60 °C and 6.5, with specific activities of 7,900 and 4,800 U/nmol, respectively.

Article Title: Genomic heterogeneity of NAD(P)H dehydrogenase predisposes Cryptosporidium to clofazimine resistance.
Article Snippet: Tag-free CpNDH2 was prepared by cleavage of MBP-CpNDH2 with factor Xa protease (New England Biolabs).

Plasmid Preparation:

Article Title: A milestone in C4 carbon concentration mechanism evolution: structural remodeling of NADP-malic enzyme in Poaceae
Article Snippet: .. For the pET16b vector, Factor Xa protease (New England Biolabs, Ipswich, USA) was used, while for the pET28b vector, Thrombin (Merck KGaA, Darmstadt, Germany) was employed. ..

Article Title: A milestone in C4 carbon concentration mechanism evolution: structural remodeling of NADP-malic enzyme in Poaceae.
Article Snippet: .. For the pET16b vector, Factor Xa protease (New England Biolabs, Ipswich, USA) was used, while for the pET28b vector, Thrombin (Merck KGaA, Darmstadt, Germany) was employed. ..

Incubation:

Article Title: Specific SLC25 carriers regulate mitochondrial protein synthesis
Article Snippet: .. The resin was resuspended with 0.7 ml of buffer B into a final volume of 1.5 ml and incubated with 7.5 μg of factor Xa protease (NEB) and on-column cleavage performed overnight at 4°C with rotation. ..

Article Title: Specific SLC25 carriers regulate mitochondrial protein synthesis.
Article Snippet: .. The resin was resuspended with 0.7 ml of buffer B into a final volume of 1.5 ml and incubated with 7.5 μg of factor Xa protease (NEB) and on- column cleavage performed overnight at 4°C with rotation. ..

Bacteria:

Article Title: The Role of Metal Complexation in the Unfolding Energetics of a Nudix Hydrolase.
Article Snippet: All chemicals, reagents, pyrophosphate assay kits, and Amicon centrifugation filters were purchased from Millipore-Sigma, Inc. (St. Louis, MO). .. Chemically competent bacteria and Factor Xa protease were obtained from New England Biolabs (Beverly, MA). .. Chromatography supplies including resins and columns were purchased from Fisher Scientific.

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